产品货号：
Z22171
中文名称：
NOXO1抗体
英文名称：
Anti-NOXO1 Antibody
产品规格：
100μg
发货周期：
1～3天
产品价格：
询价
| 抗体名称 | Anti-NOXO1 Antibody |
| 指标别名 | NOXO1;NOXO-1;NOXO 1;NADPH oxidase organizer 1;NADPH oxidase regulatory protein;Nox organizer 1;Nox organizing protein 1;Regulatory protein P41NOX;SH3 and PX domain-containing protein 5;P41NOX;SH3PXD5 |
| 克隆性 | Polyclonal |
| 检验物种 | human |
| 应用范围 | ELISA,IHC,WB |
| 基因名称 | NOXO1 |
| 抗体来源 | Rabbit |
| 抗体类型 | IgG |
| 免疫原 | NOXO1 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 238-252 of human NOXO1 protein. |
| 成分 | 0.02M Potassium Phosphate,0.15M Sodium Chloride,pH7.2,0.01% (w/v) Sodium Azide |
| 纯化方式 | Anti-NOXO-1 antibody is directed against human NOXO1 protein.The product was affinity purified from monospecific antiserum by immunoaffinity purification.?A BLAST analysis was used to suggest cross-reactivity with NOXO1 protein from human and chimpanzee (100% homology).Expect reactivity with alpha,delta and gamma isoforms of NOXO1.Also expect partial reactivity against NOXO1 homologues from dog and guinea pig (87%),as well as rat (75%) and mouse (68%).Reactivity against homologues from other sources is not known. |
| 浓度 | 1.0mg/mL by UV absorbance at 280nm |
| 产品形态 | 溶液 |
| 保存条件 | Store vial at -20℃ prior to opening.Aliquot contents and freeze at -20℃ or below for extended storage.Avoid cycles of freezing and thawing.Centrifuge product if not completely clear after standing at room temperature.This product is stable for several weeks at 4℃ as an undiluted liquid.Dilute only prior to immediate use.Expiration date is one (1) year from date of opening. |
| 背景资料 | This antibody is suitable for Cancer,Immunology and Nuclear Signaling research.The enzymes NADPH oxidase (NOX) and dual oxidase (DUOX) generate ROS in a regulated manner,producing reactive oxygen in various cells and tissues in response to growth factors,cytokines and calcium signals.The oxidase consists of the catalytic subunit gp91phox (otherwise known as NOX2),together with the regulatory subunits p22phox,p47phox,p40phox,p67phox and the small GTPase RAC.The enzyme activity of gp91phox is regulated by the assembly of these regulatory subunits with gp91phox to form an active complex.In 1999,the first of the NOX homologues of gp91phox was described as NOX1.The enzyme was cloned from a colon epithelial cell complementary DNA library.When expressed in cells,NOX1 generated low amounts of ROS,but high-level ROS production by NOX1 was subsequently achieved by co-expression with novel regulatory subunits (described later).Subsequently,NOX3 and NOX4 were cloned,and the latter was shown to generate high levels of ROS when expressed in cells.NOX organizer 1 (NOXO1) is a homologue of p47phox and has an almost identical domain organization,except that it lacks the auto-inhibitory region.NOX activator 1 (NOXA1) is a homologue of p67phox and similarly shares the same domain organization,except that it lacks one of the two SH3 domains that are present in p67phox.Co-transfection of NOX1,NOXO1 and NOXA1 results in marked ROS generation.Similar to p47phox,NOXO1 binds to p22phox,which is required for NOX1-dependent activity.NOXA1 has a well-conserved activation domain,implying a conserved mechanism for regulating the activity of the target NOX enzyme. |
| Uniprot ID | Q8NFA2 |
| 推荐稀释比 | ELISA:1:20,000 - 1:100,000 IHC:5～10μg/mL WB:1:1,000 - 1:10,000 |
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